Selective 1Hα NMR methods to reveal functionally relevant proline cis/trans isomers in IDPs: characterization of minor forms, effects of phosphorylation and occurrence in proteome

It is important to identify proline cis/trans isomers that appear in several regulatory mechanisms of proteins, and characterize minor species are present due the conformational heterogeneity intrinsically disordered proteins (IDPs). To obtain residue level information on these mobile systems we introduce two 1Hα-detected, selective, real-time homodecoupled NMR experiments analyze abundant transactivation domain p53. The measurements sensitive enough conformers 4–15 % amounts; moreover, show consequences CK2 phosphorylation cis/trans-proline equilibrium. Using our results available literature data perform a statistical analysis how amino acid type effects distribution. methods applicable under physiological conditions, they can contribute find key may help sequence optimization for biotechnological purposes.